9IQB
Crystal structure of beta-glucosidase from Acetivibrio thermocellus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-01-28 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 |
| Unit cell lengths | 109.930, 158.620, 158.910 |
| Unit cell angles | 60.12, 88.19, 87.48 |
Refinement procedure
| Resolution | 34.991 - 3.000 |
| Rwork | 0.300 |
| R-free | 0.35000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.407 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.165 | 0.650 |
| Rmeas | 0.233 | 0.920 |
| Number of reflections | 175099 | 31024 |
| <I/σ(I)> | 4.33 | |
| Completeness [%] | 93.9 | |
| Redundancy | 1.99 | |
| CC(1/2) | 0.970 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M HEPES, 55% MPD |
