9IQ5

CatM, SnoaL-like protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97919
Spacegroup name	P 21 21 21
Unit cell lengths	44.092, 61.985, 99.609
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.820 - 2.060
R-factor	0.238
Rwork	0.236
R-free	0.26970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9iq3
RMSD bond length	0.010
RMSD bond angle	1.062
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.820	2.130
High resolution limit [Å]	2.060	2.060
Number of reflections	16707	1466
<I/σ(I)>	17.92
Completeness [%]	95.1
Redundancy	11.3
CC(1/2)	0.997	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291.15	2.0 M Ammonium sulfate 0.1 M Sodium HEPES 7.5 2 % v/v PEG 400