9IQ3

AkaM,SnoaL-lile Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	74.167, 74.167, 108.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.080 - 2.500
R-factor	0.1981
Rwork	0.197
R-free	0.21640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.306
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.440	2.565
High resolution limit [Å]	2.500	2.500
Number of reflections	20398	19471
<I/σ(I)>	6.34
Completeness [%]	99.5
Redundancy	2
CC(1/2)	0.994	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291.15	0.2 M Sodium citrate tribasic dihydrate; 0.1 M Sodium HEPES 7.5; 20 % v/v 2-Propanol