Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.649, 62.233, 88.549 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.400 - 1.520 |
| R-factor | 0.1843 |
| Rwork | 0.183 |
| R-free | 0.20740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.845 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.650 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmeas | 0.057 | 1.025 |
| Rpim | 0.021 | 0.374 |
| Number of reflections | 40472 | 1970 |
| <I/σ(I)> | 21.2 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | |
| CC(1/2) | 1.000 | 0.722 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 400, ammonium sulfate, MES pH 6.5 |
