9IN1
Crystal Structure of C-terminal domain of nucleocapsid protein from SARS-CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-01 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.045, 43.486, 74.785 |
| Unit cell angles | 90.00, 94.45, 90.00 |
Refinement procedure
| Resolution | 24.050 - 1.400 |
| R-factor | 0.13388 |
| Rwork | 0.132 |
| R-free | 0.16963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6yun |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.963 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.460 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 85007 | 3753 |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 94.4 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.999 | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG4000, 0.2 M Lithium sulfate, 50 mM Tris pH 8.0 |
