9ILW

Crystal structure of the periplasmic domain of sensor protein EvgS from Escherichia coli str. K-12 substr. MG1655

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	85.560, 109.914, 118.666
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.867 - 2.700
R-factor	0.207
Rwork	0.204
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.594
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.870	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.254	1.168
Rmeas	0.270	1.237
Rpim	0.088	0.403
Number of reflections	31375	4115
<I/σ(I)>	10.7	2.8
Completeness [%]	99.9
Redundancy	9.2
CC(1/2)	0.991	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1 volume of the protein solution (18 mg/ml in 20 mM Tris/HCl, 200 mM NaCl, pH 8.0) was then mixed with 1 volume of reservoir solution (28% w/v Polyethylene glycol 4,000, 0.1 M TRIS, 0.8 M lithium chloride, pH 8.5) .