9IKF
Bovine Heart Cytochrome c Oxidase in the Carbon Dioxide-bound Fully Oxidized State
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2024-04-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 182.500, 204.400, 177.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.750 |
| R-factor | 0.13171 |
| Rwork | 0.130 |
| R-free | 0.16674 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8h8r |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.867 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmeas | 0.108 | 2.700 |
| Number of reflections | 1295927 | 96102 |
| <I/σ(I)> | 18.5 | 1.32 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.2 | 21.1 |
| CC(1/2) | 0.999 | 0.767 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.8 | 277 | 40 mM sodium phosphate buffer, pH 6.8 0.2% (w/v) decyl-maltoside 1% (w/v) polyethylene glycol 4000 |
