9IJC
Crystal structure of the beta,kappa-carrageenase Cgbk16A from Wenyingzhuangia fucanilytica
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.370, 76.833, 100.329 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.160 - 1.520 |
| R-factor | 0.1708 |
| Rwork | 0.170 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.975 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.160 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.053 | 0.255 |
| Rmeas | 0.056 | 0.266 |
| Rpim | 0.016 | 0.073 |
| Number of reflections | 55782 | 5175 |
| <I/σ(I)> | 21.5 | 2.5 |
| Completeness [%] | 97.8 | 94.1 |
| Redundancy | 12.7 | 12.9 |
| CC(1/2) | 0.999 | 0.986 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 25.5% PEG 4000, 15% Glycerol, 0.17 M Ammonium sulfate |
