9IJC

Crystal structure of the beta,kappa-carrageenase Cgbk16A from Wenyingzhuangia fucanilytica

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	46.370, 76.833, 100.329
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.160 - 1.520
R-factor	0.1708
Rwork	0.170
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.975
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.160	1.570
High resolution limit [Å]	1.520	1.520
Rmerge	0.053	0.255
Rmeas	0.056	0.266
Rpim	0.016	0.073
Number of reflections	55782	5175
<I/σ(I)>	21.5	2.5
Completeness [%]	97.8	94.1
Redundancy	12.7	12.9
CC(1/2)	0.999	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	25.5% PEG 4000, 15% Glycerol, 0.17 M Ammonium sulfate