9IIP
Fucokinase part of FKP with beta-L-fucose 1-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-11 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0000 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 95.012, 120.770, 164.979 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.020 - 2.450 |
R-factor | 0.1876 |
Rwork | 0.186 |
R-free | 0.21750 |
Structure solution method | MAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.531 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.550 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.050 | 0.966 |
Number of reflections | 17444 | 1726 |
<I/σ(I)> | 29.9 | 2.2 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 6.2 | 6.2 |
CC(1/2) | 0.942 | 0.737 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | PEG 3350, Trypton, HEPES, beta-L-fucose 1-phosphate, MnCl2 |