9IIP

Fucokinase part of FKP with beta-L-fucose 1-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-11
Detector	RAYONIX MX300HE
Wavelength(s)	1.0000
Spacegroup name	F 2 2 2
Unit cell lengths	95.012, 120.770, 164.979
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.020 - 2.450
R-factor	0.1876
Rwork	0.186
R-free	0.21750
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.531
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.550
High resolution limit [Å]	2.450	2.450
Rmerge	0.050	0.966
Number of reflections	17444	1726
<I/σ(I)>	29.9	2.2
Completeness [%]	99.6	99.9
Redundancy	6.2	6.2
CC(1/2)	0.942	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	PEG 3350, Trypton, HEPES, beta-L-fucose 1-phosphate, MnCl2