9IIA

Crystal structure of the free histidine prenyltransferase FunA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 63 2 2
Unit cell lengths	202.210, 202.210, 111.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.450 - 2.270
R-factor	0.2147
Rwork	0.213
R-free	0.25220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.030
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.480	2.330
High resolution limit [Å]	2.270	2.270
Number of reflections	61921	4522
<I/σ(I)>	24
Completeness [%]	100.0
Redundancy	42.3	37.2
CC(1/2)	0.999	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	1.5 M ammonium sulfate, 0.1 M BIS-TRIS pH 6.5, 2% v/v polyethylene glycol monomethyl ether 550