9II8
Crystal structure of Staphylococcus aureus DinG protein in complex with ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-01-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97936 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 229.170, 229.170, 128.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 111.770 - 3.160 |
| R-factor | 0.2699 |
| Rwork | 0.269 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.376 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.770 | 3.273 |
| High resolution limit [Å] | 3.160 | 3.160 |
| Rmerge | 0.055 | 0.550 |
| Number of reflections | 29417 | 2913 |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M LiSO4, 0.1 M MES (pH 6.5), and 15% Polyethylene glycol 4000 |
