9IHS
Microbial transglutaminase mutant - D3C/G283C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.760, 117.360, 85.470 |
| Unit cell angles | 90.00, 113.03, 90.00 |
Refinement procedure
| Resolution | 47.080 - 2.000 |
| Rwork | 0.181 |
| R-free | 0.23450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.579 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.659 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.037 | 0.143 |
| Number of reflections | 93774 | 22950 |
| <I/σ(I)> | 24.04 | |
| Completeness [%] | 97.1 | |
| Redundancy | 3.8288 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 25 w/v% polyethylene glycol 1000, 100 mM MES-NaOH buffer (pH5.0), and 25 mM CaCl2 |
