9IHL

Crystal Structure of the Human Nonmuscle Myosin 2A Motor Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	60.994, 120.879, 149.438
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.720 - 2.020
R-factor	0.2078
Rwork	0.205
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487: ???)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.720	2.286
High resolution limit [Å]	2.020	2.020
Rmerge	0.191	1.702
Rpim	0.054	0.491
Number of reflections	34413	1721
<I/σ(I)>	10.2	1.7
Completeness [%]	92.2	71.6
Redundancy	13.6	12.4
CC(1/2)	0.998	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	16% PEG3350, 240 mM Sodium thiocyanate