9IGP

CD177 extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-28
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.0718
Spacegroup name	H 3
Unit cell lengths	192.990, 192.990, 71.808
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	66.064 - 2.700
Rwork	0.224
R-free	0.25850
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	2.043
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	HKL2Map
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.100))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	96.920	3.010	2.830
High resolution limit [Å]	2.700	2.870	2.700
Number of reflections	27651	3071	3693
<I/σ(I)>	4.2
Completeness [%]	100.0
Redundancy	7
CC(1/2)	0.994	0.567	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% w/v PEG 3350, 0.20 M ammonium nitrate
1	VAPOR DIFFUSION, SITTING DROP		291	20% w/v PEG 3350, 0.20 M ammonium nitrate