9IGK
Crystal structure of P. syringae phosphinothricin acetyltransferase PSPTO_3321
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 105.524, 137.666, 61.903 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.390 - 1.600 |
| R-factor | 0.1878 |
| Rwork | 0.187 |
| R-free | 0.20890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.976 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.390 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.067 | |
| Rmeas | 0.071 | 123.500 |
| Rpim | 0.022 | 49.900 |
| Number of reflections | 119224 | 5781 |
| <I/σ(I)> | 15.9 | 1.3 |
| Completeness [%] | 99.8 | 97.7 |
| Redundancy | 9.8 | 5.9 |
| CC(1/2) | 0.999 | 0.647 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% (w/v) PEG 3350 and 0.2M tri-sodium citrate |
