9IF1

Unliganded structure of RNA duplex containing UGGAA/UGGAA motif

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-10
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 41 21 2
Unit cell lengths	33.818, 33.818, 170.094
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.520 - 2.790
Rwork	0.257
R-free	0.30850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.742
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.520	2.960
High resolution limit [Å]	2.790	2.790
Number of reflections	2764	348
<I/σ(I)>	16.72
Completeness [%]	97.3
Redundancy	6.26
CC(1/2)	0.999	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	0.012M Sodium chloride, 0.08M Potassium chloride, 0.04M Sodium cacodylate trihydrate pH 5.5 45% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.002M Hexammine cobalt(III) chloride