9ICX
DNA POLYMERASE BETA (POL B) (E.C.2.7.7.7) COMPLEXED WITH SIX BASE PAIRS OF DNA (NON GAPPED DNA ONLY)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-12-02 |
| Detector | SDMS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 179.010, 57.729, 48.511 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.178 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 9icw |
| RMSD bond length | 0.021 |
| RMSD bond angle | 3.000 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | TNT (V. 5-D) |
| Refinement software | TNT (5-D) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 14354 | |
| Completeness [%] | 89.0 | 80 |
| Redundancy | 1.7 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | SEE REFERENCE 1 FOR DETAILS CONCERNING EXPERIMENTAL PROCEDURES, RESULTS, AND DISCUSSION FOR THIS STRUCTURE., pH 6.50 |
