9ICW

DNA POLYMERASE BETA (POL B) (E.C.2.7.7.7) COMPLEXED WITH SIX BASE PAIRS OF DNA; NATIVE STRUCTURE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1991-09-12
Detector	SDMS
Spacegroup name	P 21 21 2
Unit cell lengths	178.638, 57.846, 48.489
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.175
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bpb 2bpg
RMSD bond length	0.021
RMSD bond angle	2.900
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	X-PLOR
Refinement software	TNT (5-D)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.063	0.202
Number of reflections	15291
Completeness [%]	95.0	81
Redundancy	2.8	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		SEE REFERENCE 1 FOR DETAILS CONCERNING EXPERIMENTAL PROCEDURES, RESULTS, AND DISCUSSIONS FOR THIS STRUCTURE., pH 6.50