9IC8
Structure of BstEII with DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978530 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.966, 203.986, 75.768 |
| Unit cell angles | 90.00, 91.76, 90.00 |
Refinement procedure
| Resolution | 101.990 - 2.720 |
| R-factor | 0.2794 |
| Rwork | 0.275 |
| R-free | 0.32450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.698 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.993 | 2.769 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.176 | 1.670 |
| Rmeas | 0.190 | 1.799 |
| Rpim | 0.072 | 0.664 |
| Number of reflections | 30279 | 1474 |
| <I/σ(I)> | 6.8 | 1.2 |
| Completeness [%] | 98.6 | 99.3 |
| Redundancy | 7 | 7.2 |
| CC(1/2) | 0.991 | 0.370 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Morpheus - Buffer System 2 pH 7.5 0.09M NPS 32.5% Morpheus - Precipitant Mix 4 |
