9IBP
VCA0144 from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-20 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.03321 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 123.400, 123.400, 226.085 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.310 - 1.800 |
| R-factor | 0.1522 |
| Rwork | 0.151 |
| R-free | 0.18220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.031 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.310 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 117266 | 7075 |
| <I/σ(I)> | 8.61 | |
| Completeness [%] | 98.2 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.996 | 0.431 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.2M Lithium Sulfate monohydrate, 0.1M Bis-TRIS PH 5.0, 25% PEG 3,350, 20% Glycerol |
