9IBN
Crystal structure of the peptidyl-prolyl isomerase (PPIase) from E. faecium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-10 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.486, 69.674, 87.413 |
| Unit cell angles | 90.00, 102.81, 90.00 |
Refinement procedure
| Resolution | 65.810 - 2.480 |
| R-factor | 0.20916 |
| Rwork | 0.207 |
| R-free | 0.25010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.928 |
| Data reduction software | XDS |
| Data scaling software | AutoProcess |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.810 | 7.958 |
| High resolution limit [Å] | 2.479 | 2.479 |
| Number of reflections | 17572 | 879 |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 86.7 | |
| Redundancy | 2.8 | |
| CC(1/2) | 0.823 | 0.020 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Sodium acetate trihydrate pH 4.6, 0.1 M Cadmium chloride hydrate, 30% v/v Polyethylene glycol 400. |
