9IB8
Tumor necrosis factor-like lectin PLTL from Photorhabdus laumondii in complex with Lewis Y tetrasaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97630 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 81.848, 81.848, 112.574 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.958 - 1.600 |
| Rwork | 0.180 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.806 |
| Data reduction software | autoPROC |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.380 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 51136 | 7323 |
| <I/σ(I)> | 27.7 | 3.7 |
| Completeness [%] | 100.0 | |
| Redundancy | 20 | |
| CC(1/2) | 1.000 | 0.888 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293.15 | PEG1500, trisodium citrate |
