9IB6

Apo form of Tumor necrosis factor-like lectin PLTL from Photorhabdus laumondii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	H 3 2
Unit cell lengths	83.501, 83.501, 105.577
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.673 - 1.200
Rwork	0.157
R-free	0.18150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.794
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP (11.7.02)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.673	1.260
High resolution limit [Å]	1.200	1.200
Number of reflections	44331	6417
<I/σ(I)>	18.9	1.7
Completeness [%]	100.0	100
Redundancy	19.7
CC(1/2)	1.000	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	293.15	sodium chloride, sodium citrate,