9IAQ
Arbitrium controls lysis-lysogeny through the activation of a small antirepressor protein in the majority of arbitrium-coding phages
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-06-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979257 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.873, 85.778, 84.164 |
| Unit cell angles | 90.00, 96.96, 90.00 |
Refinement procedure
| Resolution | 42.889 - 1.893 |
| Rwork | 0.194 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.953 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.88)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.889 | 1.926 |
| High resolution limit [Å] | 1.890 | 1.893 |
| Rmerge | 0.048 | |
| Rpim | 0.019 | 0.307 |
| Number of reflections | 42489 | 11534 |
| <I/σ(I)> | 21.4 | 2.1 |
| Completeness [%] | 99.8 | |
| Redundancy | 7 | |
| CC(1/2) | 0.999 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 16% PEG 4000, 10% 2-propanol, 100mM Hepes pH 7.5, 200mM ammonium sulfate |
