9IAC

Structure of the Argonaute-associated Cas4 family protein 1 (ACE1) from Microcystis aeruginosa (MaACE1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-21
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	77.858, 77.872, 110.453
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.230 - 2.520
R-factor	0.2628
Rwork	0.261
R-free	0.29520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.586
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.230	2.610
High resolution limit [Å]	2.520	2.520
Rmeas	0.036	0.405
Number of reflections	23331	2276
<I/σ(I)>	17.52	2.42
Completeness [%]	99.3
Redundancy	2
CC(1/2)	1.000	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	Protein solution: -10 mM HEPES-KOH pH 7.5 -250 mM KCl -1 mM DTT Reservoir solution: -0.1 M Bis-Tris pH 5.5 -0.2 M MgCl2 -23% PEG3350