9IA4
Bc8.108 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 94.380, 146.160, 84.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.290 - 2.360 |
| R-factor | 0.2213 |
| Rwork | 0.218 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.965 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.190 | 2.490 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmeas | 0.202 | 1.782 |
| Number of reflections | 24499 | 3718 |
| <I/σ(I)> | 12.71 | 1.97 |
| Completeness [%] | 99.1 | 94.7 |
| Redundancy | 13.5 | |
| CC(1/2) | 0.997 | 0.614 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292 | 10% (w/v) PEG 8K, 200 mM magnesium chloride, 100 mM Tris-HCl pH 7 |
