9I9X
Human GABARAP in complex with artificial peptide IM-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-07 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 101.340, 101.340, 101.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.370 - 1.420 |
| R-factor | 0.1747 |
| Rwork | 0.173 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.856 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.370 | 1.460 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmeas | 0.071 | 2.824 |
| Number of reflections | 32718 | 2386 |
| <I/σ(I)> | 20.49 | 1.31 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.84 | 14.13 |
| CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | ammonium sulfate, sodium cacodylate trihydrate, PEG 8000, DMSO, Tris-HCl, NaCl |
