9I9G
Crystal structure of human eIF4A1 C-terminal domain in complex with hippuristanol
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.199, 32.974, 37.781 |
| Unit cell angles | 77.09, 74.67, 81.04 |
Refinement procedure
| Resolution | 35.002 - 1.700 |
| Rwork | 0.149 |
| R-free | 0.18960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.775 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.760 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.060 | 0.173 |
| Number of reflections | 15055 | 714 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.997 | 0.967 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 20 % (w/v) PEG 6000, 0.2 M sodium chloride, 0.1 M sodium acetate, pH 5.0 |
