9I8A

NXT1-NXF1 complex crystallized in space group P65 2 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9220
Spacegroup name	P 65 2 2
Unit cell lengths	105.332, 105.332, 171.409
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	91.220 - 1.501
Rwork	0.170
R-free	0.20050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.644
Data reduction software	xia2
Data scaling software	xia2
Phasing software	SIMBAD
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.100))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.220	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.113	5.265
Number of reflections	89974	4422
<I/σ(I)>	16.6
Completeness [%]	100.0
Redundancy	37.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	3 M Sodium acetate