9I7Q

The structure of the Cep57-N.Cep63-N complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	122.009, 122.009, 69.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.990 - 2.900
R-factor	0.2465
Rwork	0.245
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.295
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.820	8.700
High resolution limit [Å]	2.900	2.900
Number of reflections	7189	1121
<I/σ(I)>	24.9	1.9
Completeness [%]	100.0	100
Redundancy	37.6	40
CC(1/2)	1.000	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	12% PEG4000, 5% PEG 1,000, 0.075M Na citrate pH=5.8, 0.075M Ammonium sulfate, 0.025M Li sulfate monohydrate, 0.025M Na citrate tribasic dihydrate pH=5.5, 15% Glycerol