9I7D
Crystal structure of wild-type HRP-2 PWWP domain - Crystal form P212121 type 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97893 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.883, 59.833, 105.778 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.890 - 2.360 |
| R-factor | 0.22084 |
| Rwork | 0.217 |
| R-free | 0.28074 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.544 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.890 | 2.490 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.054 | 0.330 |
| Rmeas | 0.063 | 0.387 |
| Rpim | 0.029 | 0.193 |
| Total number of observations | 43845 | 6021 |
| Number of reflections | 10576 | 1525 |
| <I/σ(I)> | 15.7 | 5 |
| Completeness [%] | 93.0 | |
| Redundancy | 4.1 | 3.9 |
| CC(1/2) | 0.989 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.2 M Ammonium sulfate 0.2 M Ammonium phosphate |
