9I7B
Crystal structure of HRP-2 PWWP domain with C64S mutation - Crystal form C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-24 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.086, 41.835, 72.257 |
| Unit cell angles | 90.00, 102.21, 90.00 |
Refinement procedure
| Resolution | 38.190 - 1.410 |
| R-factor | 0.18187 |
| Rwork | 0.180 |
| R-free | 0.20841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.780 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.190 | 1.490 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.146 | 2.083 |
| Rmeas | 0.159 | 2.284 |
| Rpim | 0.063 | 0.919 |
| Total number of observations | 30324 | |
| Number of reflections | 40436 | 5388 |
| <I/σ(I)> | 11.3 | 1.1 |
| Completeness [%] | 91.3 | |
| Redundancy | 6.2 | 5.6 |
| CC(1/2) | 0.996 | 0.499 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.15 M Ammonium sulfate 28% PEG MME 2000 0.1 M Sodium acetate pH 4.6 |
