9I7A
Crystal structure of HRP-2 PWWP domain with C64S mutation - Crystal form P1 type 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-24 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.127, 42.590, 44.242 |
| Unit cell angles | 62.08, 76.38, 87.00 |
Refinement procedure
| Resolution | 36.650 - 1.050 |
| R-factor | 0.16515 |
| Rwork | 0.164 |
| R-free | 0.18808 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.821 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.660 | 1.110 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.062 | 1.257 |
| Rmeas | 0.067 | 1.388 |
| Rpim | 0.025 | 0.573 |
| Total number of observations | 49819 | |
| Number of reflections | 85077 | 9337 |
| <I/σ(I)> | 14.4 | 1.4 |
| Completeness [%] | 88.6 | |
| Redundancy | 6.8 | 5.3 |
| CC(1/2) | 0.999 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.4 M ammonium acetate pH 5.0 30% PEG 4000 |
