9I6Q
Structure of Fab-Fragment GB11 agains tumour associated carbohydrate antigens.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 4 |
| Unit cell lengths | 109.160, 109.160, 40.268 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.866 - 1.860 |
| Rwork | 0.200 |
| R-free | 0.23870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.804 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.580 | 1.900 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.202 | 2.721 |
| Rmeas | 0.211 | 2.852 |
| Rpim | 0.086 | 0.847 |
| Number of reflections | 40412 | 2462 |
| <I/σ(I)> | 7.7 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.6 | 11.2 |
| CC(1/2) | 0.376 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | DL-Malic acid, Citric acid, PEG 3350 |
