9I6N

Hen egg-white lysozyme structure collected at EuXFEL SPB/SFX with HVE injection method

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	EUROPEAN XFEL BEAMLINE SPB/SFX
Synchrotron site	European XFEL
Beamline	SPB/SFX
Temperature [K]	297
Detector technology	PIXEL
Collection date	2024-08-22
Detector	PSI JUNGFRAU 4M
Wavelength(s)	0.97
Spacegroup name	P 43 21 2
Unit cell lengths	79.300, 79.300, 37.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.820 - 1.700
R-factor	0.1654
Rwork	0.162
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.386
Data reduction software	CrystFEL (0.10.1)
Data scaling software	CrystFEL (0.10.1)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.650	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	13812	1348
<I/σ(I)>	6.85
Completeness [%]	100.0	100
Redundancy	267.1	195.8
CC(1/2)	0.956	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	3	297	20% NaCl, 6% PEG 6000, 0.1M sodium acetate pH 3.0