9I5F
Glyceraldehyde 3-phosphate dehydrogenase A (GAPDHA) NAD holoenzyme, from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.6888 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 85.936, 94.866, 95.701 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.370 - 1.162 |
| Rwork | 0.136 |
| R-free | 0.16370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.989 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.370 | 1.310 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmeas | 0.130 | 1.703 |
| Number of reflections | 82091 | 4105 |
| <I/σ(I)> | 11.3 | 1.6 |
| Completeness [%] | 94.1 | 62.4 |
| Redundancy | 13.8 | 13.4 |
| CC(1/2) | 0.997 | 0.833 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 200mM sodium citrate tribasic 20% PEG 3350 (w/v) solution was mixed 4:1:1 or 3:2:1 (protein: reservoir: seeds) in 300nl drops. |
