9I5A
Crystal structure of wild type perlecan region 3 construct I876-V1272 construct including one laminin IV-like and four laminin EGF-like domains.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.7293 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.637, 74.612, 78.769 |
| Unit cell angles | 74.38, 74.08, 79.73 |
Refinement procedure
| Resolution | 46.244 - 2.045 |
| Rwork | 0.178 |
| R-free | 0.20860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.583 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.88)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.320 | 2.100 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 75120 | 5453 |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.997 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295.15 | 0.85M succinic acid, pH 7 |
