9I59
RVFV GnH complexed with RVFV-379 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-07 |
| Detector | DECTRIS EIGER2 X CdTe 9M |
| Wavelength(s) | 0.6199 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 118.090, 187.370, 157.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.470 - 2.090 |
| R-factor | 0.2139 |
| Rwork | 0.212 |
| R-free | 0.25110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.723 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.470 | 2.130 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 103314 | 5081 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.9 | |
| CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.65 | 5% PEG4000, 50 mM MES pH 6.0, 5 mM MgSO4 |
