9I22

Structure of Human helicase RecQ1- Myc G-quadruplex - ADP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978470
Spacegroup name	P 41 21 2
Unit cell lengths	155.195, 155.195, 126.841
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.600 - 2.420
R-factor	0.2206
Rwork	0.218
R-free	0.26840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.156
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.600	2.580
High resolution limit [Å]	2.420	2.420
Rmerge	0.096	1.875
Number of reflections	40883	2045
<I/σ(I)>	21.19	0.46
Completeness [%]	93.4	72.4
Redundancy	25.2	17.4
CC(1/2)	0.999	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	D-Malic acid 0.1M pH 7 PEG 3350 12%