9I08
The Paulinella chromatophore transit peptide part2 (crTPpart2) from ArgC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.069, 67.227, 43.774 |
| Unit cell angles | 90.00, 92.03, 90.00 |
Refinement procedure
| Resolution | 43.750 - 2.000 |
| R-factor | 0.1766 |
| Rwork | 0.174 |
| R-free | 0.23450 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.804 |
| Data reduction software | autoPROC (2.3.74) |
| Data scaling software | XSCALE (Jan 10, 2022) |
| Phasing software | PHASER (3.1) |
| Refinement software | PHENIX (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.750 | 2.070 |
| High resolution limit [Å] | 1.750 | 2.000 |
| Number of reflections | 14000 | 1394 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 98.8 | 98.9 |
| Redundancy | 2 | |
| CC(1/2) | 0.998 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | 23% PEG 3350 in 0.1 M HEPES pH 8.5 |
