9HZK

sdAb9 in complex with PfCSP aTSR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-07
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.967697
Spacegroup name	P 32
Unit cell lengths	53.740, 53.740, 60.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.460 - 2.070
R-factor	0.1863
Rwork	0.184
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.855
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.460	2.280
High resolution limit [Å]	2.070	2.070
Number of reflections	23186	5814
<I/σ(I)>	5.36	0.68
Completeness [%]	99.6
Redundancy	1.9
CC(1/2)	0.993	0.374

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	35% PEG4000