9HYC
Iron loaded human mitochondrial ferritin 5 minute oxygen soak
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.975 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.903, 182.903, 182.903 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.150 - 1.710 |
| R-factor | 0.1684 |
| Rwork | 0.167 |
| R-free | 0.19450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.978 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.150 | 1.772 |
| High resolution limit [Å] | 1.710 | 1.711 |
| Number of reflections | 3006218 | 2744 |
| <I/σ(I)> | 22.32 | |
| Completeness [%] | 99.7 | |
| Redundancy | 104.5 | |
| CC(1/2) | 1.000 | 0.497 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 289 | 0.1 M Bicine 2 M Magnesium chloride 0.1 M Sodium chloride 60 mM Ferrous chloride 3 mM Sodium azide |
