9HY7
AlfB fucosidase in complex with Fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 168.179, 309.559, 175.139 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.720 - 2.300 |
| R-factor | 0.17881 |
| Rwork | 0.177 |
| R-free | 0.20474 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.720 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.082 | |
| Number of reflections | 194496 | 28669 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 97.0 | 98.6 |
| Redundancy | 4.5 | 4.3 |
| CC(1/2) | 0.984 | 0.719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 3% PEG 8000, 25% MPD, 7% Taximate pH5 |
