9HY5

sc-4E (scFv derived from the mAb 4E1 against CD93) crystallized at pH 9.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97626
Spacegroup name	P 32 2 1
Unit cell lengths	64.731, 64.731, 160.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.060 - 1.600
R-factor	0.16813
Rwork	0.167
R-free	0.19950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.961
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.060	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.065	1.279
Rmeas	0.067	1.316
Rpim	0.016	0.305
Number of reflections	51826	2554
<I/σ(I)>	19.6	2.9
Completeness [%]	98.6
Redundancy	17.8	18.4
CC(1/2)	0.998	0.596

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	4.1 M NaCl, 0.1 M bicine pH 9.0