9HY3

sc-4E (scFv derived from the mAb 4E1 against CD93) crystallized at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97626
Spacegroup name	P 32 2 1
Unit cell lengths	64.531, 64.531, 160.509
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.780 - 1.620
R-factor	0.17687
Rwork	0.175
R-free	0.20673
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	2.022
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.780	1.650
High resolution limit [Å]	1.620	1.620
Rmerge	0.073	0.981
Rmeas	0.078	1.041
Rpim	0.026	0.344
Number of reflections	49253	2457
<I/σ(I)>	13.5	2.2
Completeness [%]	98.1
Redundancy	8.8	9
CC(1/2)	0.998	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	4.3 M NaCl, 0.1 M bis-tris pH 5.5