9HY2

sc-4E (scFv derived from the mAb 4E1 against CD93) crystallized at pH 7.5 (monomeric state from gel filtration)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97630
Spacegroup name	P 32 2 1
Unit cell lengths	64.420, 64.420, 161.124
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.210 - 1.700
R-factor	0.17837
Rwork	0.177
R-free	0.21148
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	2.131
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.280	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.074	1.870
Rmeas	0.076	1.917
Rpim	0.017	0.418
Number of reflections	43566	2272
<I/σ(I)>	20.7	2.1
Completeness [%]	99.9	100
Redundancy	19.9	20.8
CC(1/2)	0.999	0.437

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	4.1 M NaCl, 0.1 M HEPES pH 7.5