9HX8

Apo structure of SRSF6 RRM2 W123A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97622
Spacegroup name	P 31 2 1
Unit cell lengths	66.070, 66.070, 46.322
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.000 - 2.000
R-factor	0.2484
Rwork	0.248
R-free	0.25890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.870
Data reduction software	autoPROC (1.1.7)
Data scaling software	STARANISO (2.3.74)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.220	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	8061	583
<I/σ(I)>	14.8
Completeness [%]	98.7
Redundancy	19.9
CC(1/2)	0.996	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	0.1 M Na-Citrate, 20 % PEG 3350