9HV9
Crystal structure of Fab34 complexed with a 7-mer peptide of FMDV VP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-07 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97627 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.258, 40.679, 71.704 |
| Unit cell angles | 90.00, 94.45, 90.00 |
Refinement procedure
| Resolution | 86.370 - 1.800 |
| R-factor | 0.2021 |
| Rwork | 0.201 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.601 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.088 | |
| Rpim | 0.036 | |
| Number of reflections | 44896 | 1575 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 96.0 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.996 | 0.294 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.5, 50 % v/v PEG 400 |
