9HV8

Crystal structure of tri-specific FMDV mAb-49 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-12
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	72.009, 78.552, 100.378
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.290 - 1.720
R-factor	0.1844
Rwork	0.183
R-free	0.21130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.791
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.000	1.750
High resolution limit [Å]	1.720	1.720
Rmerge	0.066
Rpim	0.019
Number of reflections	56341	1673
<I/σ(I)>	17.4
Completeness [%]	92.1
Redundancy	12.7
CC(1/2)	1.000	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	9.1 M imidazole pH 7.0, 20 % w/v PEG 6000