9HV2

Crystal structure of tri-specific FMDV mAb-34 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-09
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	71.411, 81.468, 74.472
Unit cell angles	90.00, 116.03, 90.00

Refinement procedure

Resolution	40.730 - 1.770
R-factor	0.2095
Rwork	0.208
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.732
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.000	1.800
High resolution limit [Å]	1.770	1.770
Rmerge	0.090
Rpim	0.037
Number of reflections	72419	2781
<I/σ(I)>	11.5
Completeness [%]	97.3
Redundancy	6.5
CC(1/2)	0.998	0.307

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M CHES pH 9.5 30 % w/v PEG 3K